TransBac library
Details
?From start codon to stop codon
?Low-copy plasmid
?Expression is inducible with IPTG
?3,462 strains
?Plasmid transfer via conjugation using the oriT of the F plasmid
?Genetically modified organism
Utility
Low copy number, allowing for expression close to natural levels
? Useful for studying essential genes
Addressing potential issues with ASKA clones
Problems caused by the addition of a His tag
Issues during transformation